Keywords
povidone iodine
blood agar plates
aerosols
colony forming units
Abstract
Background: Operator safety during dental & periodontal treatment is a non-negotiable necessity. The production of airborne material, during dental procedures is obvious to the dentist, dental team and the patient.
Aim: The aim of the present study was to evaluate, the colony forming units (CFU) generated from aerosol during ultrasonic procedure in gingivitis and periodontitis subjects that act as a potential risk factor for spread of infectious agents for both operator and patients.
Materials and Methods: The present study included 18 subjects which were randomly assigned into 3 equal groups. Group I (Control group) subjects were treated with ultrasonic scaling alone, whereas; Groups II & Group III (Test groups) subjects used pre-procedural mouthrinses before scaling & root planing (Chlorhexidine and Povidone Iodine). Blood agar plates were used to assess the aerosol contamination and were placed at operator’s eye level, subject’s eye & chest level. These plates were then incubated for 72 hours and microbial growth were quantified as colony forming units (CFU/plate). Different colonies were identified by standard biochemical methods.
Results: This study showed that the antiseptic mouthrinses significantly reduce the bacterial CFU in the aerosol. Povidone Iodine was found to be superior to Chlorhexidine when used pre-procedurally.
Conclusion: The following conclusion was drawn that the use of pre-procedural rinses significantly reduced the aerosol contamination and hence chances of cross-infection in the dental units.
Key Words: Chlorhexidine, povidone iodine, blood agar plates, aerosols, colony forming units
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