Keywords
hydrogen peroxide
plaque
propolis
Abstract
Background: Plaque is the soft deposit that forms the biofilm consisting of microorganisms adhering to the tooth surface and is proved beyond doubt to be the initiator of gingival and periodontal disease. Plaque control is the mainstay in periodontal disease.
Aim: To compare the effect of 0.2% chlorhexidine gluconate, raw propolis and 3% hydrogen peroxide mouthwash on dental plaque and gingival inflammation.
Materials and Methods: Thirty subjects in the age group of 20-40 years were enrolledin the study. Plaque index and Modified Gingival Index were recorded at baseline and oral prophylaxis was performed. Subjects were then randomly divided into three groups (n=10) and were asked to rinse with 10ml mouthwash twice daily for 15 days. Group I received 0.2% chlorhexidine gluconate mouth wash, Group II raw propolis diluted with distilled water (1:1), and Group III 3% hydrogen peroxide (1:1) mouthwash. Subjects were recalled on 7 day and 28 day for reevaluation and recording plaque index and modified gingival index. Statistical analysis was done to evaluate the efficacy of all the three mouthwashes.
Results: The results showed that all the three mouthwashes were effective in reducing plaque and gingival inflammation. Chlorhexidine gluconate 0.2% showed to be the most effective in reduction of dental plaque. Propolis was observed to be the most effective in reducing gingival inflammation over a period of 28 days.
Conclusion: Propolis can be effectively used as a mouthwash without any potential side effects as an alternative to chlorhexidine mouthwash in reducing gingival inflammation.
Keywords: Chlorhexidine gluconate; hydrogen peroxide; plaque; propolis.
References
Jan AM, Verkade H, Timmerman MF, Van der Weijden FA. Comparison of two commercial chlorhexidine mouthwashes. J Periodontol. 2003;74:214–8.
Lindhe J, Karring T, Lang NP. Oxford: Blackwell Munksgaard; Clinical Periodontology and Implant Dentistry. 2003;4th edition:Chapter 7:198.
Francesso AG, Sampathkumar P, Beatrice E. Microbiological and clinical effects of chlorhexidine digluconate and hydrogen peroxide mouthrinse osn developing plaque and gingivitis. J Clin Periodontol. 1988;15:60–7.
Fischman SL. History of oral hygiene products. How far we have come in 6000 years? Periodontol 2000. 1997;15:7–14.
Turesky S, Gilmore ND, Glickman I. Reduced plaque formation by the chloromethyl analogue of vitamin C. J Periodontol. 1970;41(1):41-3.
Lobene RR, Mankodi SM, Ciancio SG, Lamm RA,Charles CH, Ross NM. Correlations among gingival indices. A methodology study. J Periodontol. 1989;60:159-62.
Denton GW. Chlorhexidin. InS.S. Block (ed.), Disinfection, sterilization and preservation, Lea and Febiger, Philadelphia, Pa. 1991;4:274-89.
Davie GE, Francis J, Martin AR, Rose FL, Swain G. 1:6-Di 4-chlorophenyldiguanidohexane (“Hibitane”). Laboratory investigation of a new antibacterial agent of high potency. Br J Pharmacol. 1954;9:192-6.
Hugo WB, Longworth AR. The effect of chlorhexidine on the electrophoretic mobility, cytoplasmic constituents, dehydrogenase activity and cell walls of Escherichia coli and Staphylococcus aureus. J Pharm Pharmacol. 1966;18:569-78.
Hastings DC. Non-antibiotic plaque chemotherapy. In HN. Newman and M. Wilson. Dental plaque revisited: oral biofilms in health and disease. Bioline Press, Cardiff, United Kingdom. 2000;523-48.
Bonesvoll P, Lokken P, Rölla G, Paus PN. Retention of chlorhexidine in the human oral cavity after mouthrinses. Arch Oral Biol. 1974;19:1209-12.
Hase JC, Edwardsson S, Rundegren J, Attstrom R, Kelty E. Six-month use of 0.2% delmopinol hydrochloride in comparison with 0.2% chlorhexidine digluconate and placebo. II. Effect on plaqueandsalivarymicroflora. J Clin Periodontol. 1998;25:841-9.
Rosin M, Welk A, Kocher T, Majic-Todt A, Kramer A, Pitten FA. The effect of a polyhexamethylenebiguanide mouth rinse compared to an essential oil rinse and a chlorhexidine rinse on bacterial counts and 4-day plaque re-growth. J Clin Periodontol. 2002;29:392-9.
Muyzer G, Smalla K. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Leeuwenhoek. 1998;73:127-41.
Löe H, Schiött CR. The effect of mouthrinses and topical application of chlorhexidine on the development of dental plaque and gingivitis in man. J Peridontal Res. 1970;5:79-83.
Emilson CG. Potential efficacy of chlorhexidine against mutans streptococci and human dental caries. J Dent Res. 1994;73:682-91.
Murray MC, Worthington HV, Blinkhom AS. A study to investigate the effect of a propolis-containing mouthrinse on the inhibition of de novo plaque formation. J Clin Periodontol. 1997;24(11):796-8.
Mundo MA, Padilla-Zakour OI, Worobo RW. Growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. Int J Food Microbiol. 2004;97:1-8.
Donna BL, Grundeman LJ, Steinfort J, Timmerman MF, van der Weijden GA. The inhibitory effect of combining chlorhexidine and hydrogen peroxide on 3 day plaque accumulation. J Clin Periodontol. 1998;25:879-83.
Grundeman LJ, Timmerman MF. Stain and plaque reduction by combining chlorhexidine and perborate. J Clin Periodontol. 2000;27:9-15.
Nayak PA, Nayak UA, Mythili R. Effect of Manuka honey, chlorhexidine gluconate and xylitol on the clinical levels of dental plaque. Contemp Clin Dent. 2010;4:214-7.
Gupta D Bhaskar, DJ, Jain A, Agali C, Gupta V, Kumar R, Yadav P, Lavate AB, Chaturvedi M. Comparative evaluation of honey, chlorhexidine gluconate (0.2%) and combination of xylitol and chlorhexidine mouthwash (0.2%) on the clinical level of dental plaque: A 30 days randomized control trial. Clin Res. 2015;6(1):53-7.
Bhardwaj VK, Jhingta P, Bhardwaj A, Sharma D, Kumar N, Vaid S.Effect of hydrogen peroxide mouthwash as an adjunct to chlorhexidine on stains and plaque. J Indian Soc Periodontol 2013;17(4):449-53.
Gusberti FA, Sampathkumar P, Siegrist BE, Lang NP. Microbiological and clinical effects of chlorhexidine digluconate and hydrogen peroxide mouthrinses on developing plaque and gingivitis. J Clin Periodontol. 1988;15(1):60-7.
Singh N, Charde P, Bhongade ML. Comparative Evaluation between Honey and Chlorhexidine Gluconate on the Dental Plaque Levels and Gingival Health. Adv Dent Oral Health Res. 2016;1(5)
Abbas DK, Thrane P, Othman SJ. Effectiveness of veadent as plaque-inhibiting mouthwash. Scand J Dent Res. 1985;93(6):494-7.
Abdullah BA, Al-Talib RA, Al-Sultan FA. Comparative antiplaque activity of propolis extract and chlorhexidene in vitro. Al-Rafidain. Dent J. 2003; 3(1):7-12.