Stem Cells in Dentistry: Knowledge among the Dental Practitioners of Nepal


Dental education
stem cells


Introduction: Dentists are expected to play a major role in advancing the field of stem cell research as well as directing and implementing future stem cell therapies in dentistry. Accordingly, good basic knowledge and a positive attitude toward stem cells and their potential applications are essential among the dentists.

Objective: This study aimed to assess the prevalence of knowledge on stem cell among the dental practitioners.

Methods: A descriptive cross-sectional study was conducted among the registered dental practitioners in Nepal. An online questionnaire was created with the help of Google forms between 27 October, 2021 to 22 July, 2022 after receiving ethical approval from the Institutional Review Committee of a tertiary care center. The questionnaire was designed after reviewing pertinent literatures. Convenience sampling method was used. Data from Google forms were entered in Microsoft Excel version 2016 and were analysed with IBM SPSS v.20.

Results: Among the total of 280 responses received, the prevalence of good knowledge about stem cells was found to be 53 (18.93%) (14.34- 23.52, 95% Confidence Interval). Majority of the respondents with good knowledge score were female 41 (77.36%). Almost all of the respondents knew about the term stem cells and its sources. But very few 68 (24.29%) of them knew about the availability of stem cell banks in Nepal.

Conclusions: The prevalence of good knowledge about stem cell in dentistry was found to be less than half the sample size. Hence, there is a need to cover stem cells related information in dental curricula in Nepal.



Nuti N, Corallo C, Chan BM, Ferrari M, Gerami-Naini B. Multipotent Differentiation of Human Dental Pulp Stem Cells: A Literature Review. Stem Cell Rev Rep. 2016;12(5):511-23.
Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000;97(25):13625-30.
Alhadlaq A, Al-Maflehi N, Alzahrani S, AlAssiri A. Assessment of knowledge and attitude toward stem cells and their implications in dentistry among recent graduates of dental schools in Saudi Arabia. Saudi Dent J. 2019;31(1):66-75.
Sede MA, Audu O, Azodo CC. Stem cells in Dentistry: knowledge and attitude of Nigerian Dentists. BMC Oral Health. 2013;13(1):1-8.
Jose N. Assessment of knowledge, attitude, and practice regarding applications of stem cells in dentistry among dental house surgeons, postgraduate students, and teaching faculties in two dental colleges in Ernakulam, Kerala, India. Int J Oral Care Res. 2018;6(1):65-8.
Bloom BS. Learning for mastery. Instruction and curriculum. regional education laboratory for the carolinas and virginia, topical papers and reprints, number 1. Eval Comment. 1968;1(2):1-11.
Tork HMM, Alraffaa SM, Almutairi KJ, Alshammari NE, Alharbi AA, Alonzi AM. Stem cells: knowledge and attitude among health care providers in Qassim region, KSA. Int J Adv Nurs Stud. 2018;7(1):1-7.
Akram Rahavi Ez-Abadi, Arezoo Ebn Ahmady, Fahimeh S. Tabatabaei. Dental residents’ knowledge and attitude towards stem cells and regenerative dentistry. J Dent Sch. 2017;35(3):99-107.
Shrestha RM, Shrestha S, Kunwar N. Dentists in Nepal: A Situation Analysis. J Nepal Health Res Counc. 2017;15(2):187-92.
Yu D, Silva GA. Stem cell sources and therapeutic approaches for central nervous system and neural retinal disorders. Neurosurg Focus. 2008;24(3-4):E11.
Scadden DT. The stem-cell niche as an entity of action. Nature. 2006;441(7097):1075-9.
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, et al. Embryonic Stem Cell Lines Derived from Human Blastocysts. Science. 1998;282(5391):1145-7.
Mead B, Logan A, Berry M, Leadbeater W, Scheven BA. Concise review: dental pulp stem cells: A novel cell therapy for retinal and central nervous system repair. Stem Cells. 2017;35(1):61-7.
Yen AH, Sharpe PT. Stem cells and tooth tissue engineering. Cell Tissue Res. 2008;331(1):359-72.
Bhatt R, Bhatt A, Gurjar D, Dave L. Evaluating awareness on dental pulp stem cells and its applications amongst graduating dental students of Ahmedabad and Gandhinagar district: A cross-sectional survey. Adv Hum Biol. 2014;4(3):54-9.
Saud B, Malla R, Shrestha K. Stem Cell Therapy in Nepal Challenges and Opportunities. J Stem Cell Res Dev Ther. 2019;5:022.
Sreenivas SD, Rao AS, Satyavani SS, Reddy BH, Vasudevan S. Where will the stem cells lead us? Prospects for dentistry in the 21st century. J Indian Soc Periodontol. 2011;15(3):199-204.
Park YJ, Cha S, Park YS. Regenerative applications using tooth derived stem cells in other than tooth regeneration: A literature review. Stem Cells Int. 2016;2016:e9305986.
Garcia-Godoy F, Murray PE. Status and potential commercial impact of stem cell-based treatments on dental and craniofacial regeneration. Stem Cells Dev. 2006;15(6):881–7.