Evaluation of Bovine Derived Xenograft Combined with Bioresorbable Collagen Membrane in Treatment of Intrabony Defects


Bioabsorbable collagen membrane
bovine-derived xenograft
intrabony defects
open flap debridement


Introduction: Treatment of periodontal diseases done by surgical therapy depends upon extent and severity of disease. The ultimate goal of periodontal reconstructive surgery is to regenerate tissues destroyed during periodontal disease.

Objective: To evaluate the effectiveness of bovine-derived xenograft with collagen membrane in treatment of intrabony defects by comparing it with open flap debridement alone.

Methods: This non-randomised controlled trial was conducted after ethical clearance, at Bir hospital from 2018 March to 2019 April. The study recruited 38 patients by convenience sampling, age from 25-44 years, with chronic periodontitis, and willing to sign informed consent. Intrabony defects were treated by open flap debridement with bovine-derived xenograft and bioresorbable collagen membrane (Test group) and open flap debridement alone (Control group). Probing pocket depth, clinical attachment level, gingival recession, oral hygiene status, and gingival status were assessed at baseline and six months.

Results: Six months after therapy, in Test group probing pocket depth reduction was 5.2 mm and gain in mean clinical attachment level was 4.3 mm. In Control group, mean probing pocket depth reduction was 3.8 mm and mean gain in clinical attachment level was 2.7 mm. The test treatment resulted in statistically higher probing pocket depth reduction and clinical attachment level gain than Control group.

Conclusion: Both therapies resulted in significant probing pocket depth reductions and clinical attachment gains, and treatment with open flap debridement with bovine-derived xenografts and collagen membrane resulted in significantly higher probing pocket depth reduction and clinical attachment gain than treatment with open flap debridement alone.



Page RC, Kornman KS. The pathogenesis of human periodontitis: An introduction. Periodontol 2000. 1997;14:9-11.

Cortellini P, Prato PG, Tonetti, M. The simplified papilla preservation flap. A novel surgical approach for the management of soft tissues in regenerative procedures. Int J Periodont Rest. 1999;19(6):589-99.

Bouchard P, Giovannoli JL, Mattout C, Davarpanah M, Etienne D. Clinical evaluation of a bioabsorbable regenerative material in mandibular class II furcation therapy. J Clin Periodontol. 1997;24:511-8.

Yamada S, Shima N, Kitamura H, Sugito H. Effect of porous xenographic bone graft with collagen barrier membrane on periodontal regeneration. Int J Periodont Rest. 2002;22:389-97.

Camelo M, Nevins ML, Schenk RK, Simion M, Rasperini G, Lynch SE, et al. Clinical, radiographic, and histologic evaluation of human periodontal defects treated with Bio-Oss and Bio-Gide. Int J Periodont Rest. 1998;18:321-31.

Richardson CR, Mellonig JT, Brunsvold MA, McDonnell HT, Cochran DL. Clinical evaluation of Bio-Oss: a bovine-derived xenograft for the treatment of periodontal osseous defects in humans. J Clin Periodontol. 1999;26:421-8.

Sculean A, Berakdar M, Chiantella GC, Donos N, Arweiler NB, Brecx M. Healing of intrabony defects following treatment with a bovine-derived xenograft and collagen membrane. J Clin Periodontol. 2003;30:73-80.

Ratka-Kruger P, Neukranz E, Raetzke P. Guided tissue regeneration procedure with bioresorbable membranes versus conventional flap surgery in the treatment of infrabony periodontal defects. J Clin Periodontol. 2000;27:120-7.

Starvopoulos A, Karring T. Guided tissue regeneration combined with a deproteinized bovine bone mineral (Bio-Osss) in the treatment of intrabony periodontal defects: 6-year results from a randomized-controlled clinical trial. J Clin Periodontol. 2010;37(2):200-10.

Cortellini P, Carnevale G, Sanz M, Tonetti MS. Treatment of deep and shallow intrabony defects. A multicenter randomized controlled clinical trial. J Clin Periodontol. 1998;25(12):981-7.

Mellonig JT. Human histologic evaluation of a bovine-derived bone xenograft in the treatment of periodontal osseous defects. Int J Periodontics Restorative Dent. 2000;20(1):19-29.

Caton J, Nyman S, Zander H. Histometric evaluation of periodontal surgery. II. Connective tissue attachment levels after four regenerative procedures. J Clin Periodontol. 1980;7(3):224-31.

Sculean A, Donos N, Brecx M, Reich E, Karring T. Treatment of intrabony defects with enamel matrix proteins and guided tissue regeneration. An experimental study in monkeys. J Clin Periodontol. 2000;27:466-72.

Sculean A, Windisch P, Chiantella GC, Donos N, Brecx M, Reich E. Treatment of intrabony defects with enamel matrix proteins and guided tissue regeneration. A prospective controlled clinical study. J Clin Periodontol. 2001;28:397-403.

Luepke PG, Mellonig JT, Brunsvold MA. A clinical evaluation of a bioresorbable barrier with and without decalcified freeze-dried bone allograft in the treatment of molar furcations. J Clin Periodontol. 1997;24(6):440-6.